Journal: FEMS Microbiology Letters

Article Title: Giant linear plasmids of β-lactam antibiotic producing Streptomyces

doi: 10.1111/j.1574-6968.1995.tb07749.x

Figure Lengend Snippet: Fig. 1. CHEF gel electrophoresis of DNA preparations prepared by in situ lysis of @-la&m producing Streptomyces spp. Lanes 1-7: (1) S. canleya; (2) S. chuligerus; (3) S. grisew; (4) S. jumonjinensis; (5) S. lipmannii; (6) S. iividans 1326; (7) h ladder molecular mass marker (New England Biolabs). Numbers to the side of the figures indicate molecular mass marker positions (size in kb), C indicates zone of compression.

Article Snippet: Pulsed field gel electrophoresis GLPs were separated using a Bio-Rad CHEF DR II apparatus [13] capable of resolving DNA fragments up to at least 2 Mb in size.

Techniques: Nucleic Acid Electrophoresis, In Situ, Lysis, Marker

Journal: FEMS Microbiology Letters

Article Title: Giant linear plasmids of β-lactam antibiotic producing Streptomyces

doi: 10.1111/j.1574-6968.1995.tb07749.x

Figure Lengend Snippet: Fig. 2. Sucrose gradient fractionation of linear plasmid DNA from a proteinase K high molecular mass DNA preparation of S. clauuligerus. S. clauuligerus DNA was fractionated and electrophoresed in a CHEF gel as described in the materials and methods, and stained with ethidium bromide. Lanes 1 and 20 are A ladder/A Hind111 molecular mass markers, lanes 2 to 19 correspond to sucrose gradient fractions, with lane 2 being the top of the gradient, lane 19 the bottom. Numbers to the side of the figures indicate molecular mass marker positions (sizes in kb.)

Article Snippet: Pulsed field gel electrophoresis GLPs were separated using a Bio-Rad CHEF DR II apparatus [13] capable of resolving DNA fragments up to at least 2 Mb in size.

Techniques: Fractionation, Plasmid Preparation, Staining, Marker

Journal: FEMS Microbiology Letters

Article Title: Giant linear plasmids of β-lactam antibiotic producing Streptomyces

doi: 10.1111/j.1574-6968.1995.tb07749.x

Figure Lengend Snippet: Fig. 3. Southern transfer and hybridization analysis of total cellular DNA in situ preparations of 8. clauuligerus, S. gri.seus, S. jumonjinensis, and 5. liuidans 1326 with linear plasmid probes. A: Ethidium bromide stained CHEF gel mn under the same conditions as in Fig. 1, with the DNA samples: (1) A concatamer ladder molecular mass markers; (2) 5. clauuligerus; (3) S. griseus; (4) S. jumonjinensis; and (5) 5. liuidans 1326. Panels B to H represent autoradiographs of the gel shown in panel A after hybridizations with random primer labeled probes: (B) pSCL1; (0 pSCL2; (D) pSCL3; (El pSGL1; (F) pSJL3; (G) pSJL4; and_(H) SLP2. Scale and lane contents remain constant between panels. Numbers to the side of the figures indicate molecular mass marker positions (size in kb), C indicates zone of compression.

Article Snippet: Pulsed field gel electrophoresis GLPs were separated using a Bio-Rad CHEF DR II apparatus [13] capable of resolving DNA fragments up to at least 2 Mb in size.

Techniques: Hybridization, In Situ, Plasmid Preparation, Staining, Labeling, Marker